arp 100  (TargetMol)

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: Correlation between MMP2 expression and clinicopathological features of CRC patients T: tumor depth; N: lymph node involvement; M: metastasis; MMP2: matrix metalloproteinase-2; CRC: colorectal cancer * Based on Chi-square test, or (if not applicable) Fisher’s exact test

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Expressing

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: Differences in MMP2 expression between cancerous and cancerous-adjacent normal tissues * Based on Chi-square test, or (if not applicable) Fisher’s exact test; MMP2: matrix metalloproteinase-2

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Expressing

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: The representative image showed elevated MMP2 expression levels in (A) cancerous tissues compared to those in (B) normal tissues (20x). MMP2: matrix metalloproteinase-2

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Expressing

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: MMP2 was inhibited in SW480 cells by treating with 5 μM of the MMP2 inhibitor ARP100 for 24h, while control cells were treated with DMSO. (A-C) Transwell migration and invasion assays (magnification: 100×) demonstrated that MMP2 inhibition significantly reduced the migratory and invasive capabilities of SW480 cells. (D and E) Wound healing assays (scale: 100 µm) showed that MMP2 inhibition markedly decreased gap closure in SW480 cells. Data are presented as mean ± standard error (**P≤0.002; ***P<0.0005). (F) Western blot analysis confirmed MMP2 inhibition. Results are representative of three independent experiments. MMP2: matrix metalloproteinase-2; CRC: colorectal cancer; DMSO: dimethyl sulfoxide

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Control, Migration, Inhibition, Western Blot

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: MMP2 was inhibited in SW480 cells by treating with 5 μM of the MMP2 inhibitor ARP100, while control cells were treated with DMSO. Cell proliferation was assessed using the MTT assay, with absorbance readings taken at 570 nm. (A) APR100 inhibitor significantly inhibited SW480 cell proliferation. (B and C) Transfection efficiency was validated through RT-PCR and western blot analysis. Data are presented as mean ± standard error (*P<0.02 and **P≤0.009). Results from three representative experiments are depicted. MMP2: matrix metalloproteinase-2; DMSO: dimethyl sulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide; RT-PCR: reverse transcription-polymerase chain reaction

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Control, MTT Assay, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Reverse Transcription, Polymerase Chain Reaction

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: Caspase 3 and 9 activities in SW480 treated cells were quantified using spectrophotometry. The activity of caspases in control cells (treated with DMSO) was set as 1, and the activity values of the experimental group were standardized relative to this. (A) SW480 cells treated with inhibitor exhibited a significant elevation in both caspase 3 and caspase 9 activities. (B) Western blot analysis was performed to detect the expression levels of apoptosis-related proteins. Bar graphs depict the standard errors of the mean from three experiments (**P≤0.005 or P=0.001). MMP2: matrix metalloproteinase-2; DMSO: dimethyl sulfoxide

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Spectrophotometry, Activity Assay, Control, Western Blot, Expressing